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Doug Barrick
Professor, Biophysics


Vincent J. Hilser
Professor, Biology


Katherine Tripp, Ph.D.
Center Manager

Johns Hopkins University
3400 N. Charles Street
Baltimore, MD 21218
(410) 516-3598 office
(410) 516-4118 fax

Isothermal Titration Calorimetry

  Isothermal Titration Calorimetry (ITC) is a technique that directly measures the heat released or absorbed during a biomolecular binding event.  Measurement of this heat enables accurate determination of binding constants, reaction stoichiometry, enthalpy and entropy, thereby providing complete information of the molecular interaction in a single experiment.  ITC is the method of choice for characterizing biomolecular interactions. 


ITC instructions

ITC sample preparation

ITC review


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