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Co-Directors

Doug Barrick
Professor, Biophysics
barrick@jhu.edu

 

Vincent J. Hilser
Professor, Biology
hilser@jhu.edu

 



Katherine Tripp, Ph.D.
Center Manager

Johns Hopkins University
3400 N. Charles Street
Baltimore, MD 21218

tripp@jhu.edu
(410) 516-3598 office
(410) 516-4118 fax

  
Isothermal Titration Calorimetry

      
 
 
  Isothermal Titration Calorimetry (ITC) is a technique that directly measures the heat released or absorbed during a biomolecular binding event.  Measurement of this heat enables accurate determination of binding constants, reaction stoichiometry, enthalpy and entropy, thereby providing complete information of the molecular interaction in a single experiment.  ITC is the method of choice for characterizing biomolecular interactions. 

 

ITC instructions

ITC sample preparation

ITC review
reference

 
 

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