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Co-Directors

Doug Barrick
Professor, Biophysics
barrick@jhu.edu

 

Vincent J. Hilser
Professor, Biology
hilser@jhu.edu

 



Katherine Tripp, Ph.D.
Center Manager

Johns Hopkins University
3400 N. Charles Street
Baltimore, MD 21218

tripp@jhu.edu
(410) 516-3598 office
(410) 516-4118 fax

  
Fluorescence Spectroscopy

     
  Intrinsic fluorescence or site-specific labels can be used to monitor oligomerization, ligand binding, folding and local conformational rearrangements.  Fluorescence resonance energy transfer between suitable pairs of fluorophores gives information on their distance. Excitation and emission polarisers allow for anisotropy measurements.
 

 

 

Fluorimeter instructions

 

 

 

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