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Co-Directors

Doug Barrick
Professor, Biophysics
barrick@jhu.edu

 

Vincent J. Hilser
Professor, Biology
hilser@jhu.edu

 



Katherine Tripp, Ph.D.
Center Manager

Johns Hopkins University
3400 N. Charles Street
Baltimore, MD 21218

tripp@jhu.edu
(410) 516-3598 office
(410) 516-4118 fax

  
Analytical Ultracentrifugation

 

 

   
 

Image of Beckman AUC

  

Analytical Ultracentrifugation (AUC) is the method of choice for determination of the molecular weight of macromolecular complexes (from small polypeptides to viruses) under native solution conditions.  The radial distribution of protein in the spinning cell, that arises from the action of both the outward centrifugal force and random diffusion, is mass and shape dependent (in a sedimentation velocity experiment).  Analysis of data can determine even very weak association constants and reveal the size distributions of solutions containing multiple species.

Our Beckman XL-I has absorption as well as interference optics able to observe sedimenting species under a wide range of buffer conditions and concentrations.

 


 

AUC cell components

AUC sample preparation

AUC data analysis

 

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